UCPH as a leader in Next Generation designer organisms
The use of a novel and revolutionary genome editing technology has the power to redesign most living organisms with exquisite precision, speed and cost-effectiveness. The technologies have immense translational potential in diverse areas of biotechnology and biomedicine including drug discovery, stem cell and gene therapies, livestock, and crops, but also gives rise to serious ethical and legal implications. The vision is to transform University of Copenhagen into a leading knowledge center on precise genetic engineering of cells and organisms using novel and revolutionary nuclease-based technologies.
Yang Z, Wang S, Halim A, Schulz MA, Frodin M, Rahman SH, Vester-Christensen MB, Behrens C, Kristensen C, Vakhrushev SY, Bennett EP, Wandall HH, Clausen H.
Engineered CHO cells for production of diverse, homogeneous glycoproteins.
Nat Biotechnol. 2015 Aug;33(8):842-4
Bagdonaite I, Nordén R, Joshi HJ, Dabelsteen S, Nyström K, et al. (2015)
A Strategy for O-Glycoproteomics of Enveloped Viruses—the O-Glycoproteome of Herpes Simplex Virus Type 1 . PLoS Pathog 11(4): e1004784
Prakash Radhakrishnana1, Sally Dabelsteenb,c1, et al.
Immature truncated O-glycophenotype of cancer directly induces oncogenic features
PNAS, 2014; 111(39):E4066-75.
Also see comment in Proc Natl Acad Sci U S A. 2014 Sep 30;111(39):14009-10. Not so sweet malignant transformation. Baenziger JU.
Gene editing:Advising advice Courtney Addison1 and Samuel Taylor-Alexander2 1Department for Food and Resource Economics, The University of Copenhagen, Copenhagen, 1958, Denmark. 2Mason Institute for Medicine, Life Science and the Law, School of Law, University of Edinburgh, Edinburgh, EH8 9YL, UK. Science, aug 28 2015; 349(6251); 935
Yang Z, Steentoft C, Hauge C, Hansen L, Thomsen AL, Niola F, Vester-Christensen MB, Frödin M, Clausen H, Wandall HH, Bennett EP
Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. 2015 May 19;43(9):e59
Duda K, Lonowski LA,
Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett
EP, Wandall HH, Davis GD, Hansen SH, Frödin M.
High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs. Nucleic Acids Res. 2014 Jun;42(10):e84.